This involves determining the rate of sperm DNA fragmentation by means of molecules marked with fluorescents. This technique is commonly known as TUNEL (Terminal dUTP Nick-End Labeling). FIVMadrid was the first clinic to study nuclear fragmentation using TUNEL (National Spanish Fertility Society Conference. 2001).
Studies have demonstrated that males with reproductive dysfunction have a higher proportion of sperm with DNA breaks and that this fact may have a negative impact on assisted reproduction results (implantation failures and miscarriages). However, these results are not supported by scientific evidence. Therefore, at FIVMadrid we do not use this method on a regular basis, given its limited prognostic value.